Control by Heat and UV Lab Report Example | Topics and Well Written Essays - 500 words

Control by Heat and UV - Lab Report ExampleSome bacteria though have developed reconciling measures to sustain towering-temperature exposures, for example, T. aquaticus that is used in PCR. Psychrophiles are those that require low temperatures of less than 20C while mesophiles require 20-40C. Thermophiles, on the other hand, require high temperatures of above 40C to survive. Most bacteria that infect human are mesophiles and grow optimally at 35-37C. Bacteria that have endospores are much likely to survive extreme environmental conditions such as high temperature and high UV irradiation (Prescott, John and Donald, 54)Three TSA plates were acquired and labeled with a name, lab cartridge clip, and temperature. Each was divided into three sections. Each section was marked with the abbreviation of the test organisms. A single streak (about 1cm) of each organism was made on its respective section. They were incubated at temperatures of 35C incubator, Room temperature (or 25C incubato r) and Refrigerator (10C).Four TSA plates were acquired for each bacterium. Each was labeled with the name, lab time and the organism name. The plates were then divided into half each and labeled control and UV on each side. A sterile swab was inoculated into the culture. Three zig-zag lines were made on the agar surface at 45 degrees to each other. The plates were exposed to UltraViolet light for 3 minutes before incubation at 35C for 48 hours.Four Trypticase Soy Broth tubes were labeled with name, lab time, organism and time. Each tube was inoculated with the appropriate organism. Each student in the team exposed the organism to different temperature of 40C, 55C, 80C and 100C. Each organism was exposed to heat for a stipulation length of time, that is, 10, 20, 30 and 40 minutes. The tubes were vortexed after every 10 minutes. After the appropriate length of time, the tubes were dried and incubated at 35C for 48 hours.The iv types of bacteria exhibited different responses to